氨基酸
溃疡性结肠炎
化学
炎症性肠病
三氯乙酸
气相色谱-质谱法
生物化学
内科学
医学
质谱法
色谱法
疾病
作者
Makoto Ooi,Shin Nishiumi,Tomoo Yoshie,Yuuki Shiomi,Michitaka Kohashi,Ken Fukunaga,Shirô Nakamura,Takayuki Matsumoto,Naoya Hatano,Masakazu Shinohara,Yasuhiro Irino,Tadaomi Takenawa,Takeshi Azuma,Masaru Yoshida
标识
DOI:10.1007/s00011-011-0340-7
摘要
The roles that amino acids play in immunity and inflammation are well defined, and the relationship between inflammatory bowel disease (IBD) and certain amino acids has recently attracted attention. In this study, the levels of amino acids and trichloroacetic acid (TCA) cycle-related molecules in the colonic tissues and sera of patients with ulcerative colitis (UC) were profiled by gas chromatography/mass spectrometry (GC/MS), with the aim of evaluating whether the clinical state induced by UC leads to variations in the amino acid profile.Colonic biopsy samples from 22 UC patients were used, as well as serum samples from UC patients (n = 13), Crohn's disease (CD) patients (n = 21), and healthy volunteers (n = 17).In the GC/MS-based profiling of amino acids and TCA cycle-related molecules, lower levels of 16 amino acids and 5 TCA cycle-related molecules were observed in the colonic lesion tissues of the UC patients, and the serum profiles of amino acids and TCA cycle-related molecules of the UC patients were different from those of the CD patients and healthy volunteers.Our study raises the possibility that GC/MS-based profiling of amino acids and TCA cycle-related molecules is a useful early diagnostic tool for UC.
科研通智能强力驱动
Strongly Powered by AbleSci AI