Physical and Chemical Characteristics of Glycopeptide from Wood Decay Fungi

A low-molecular-weight (7,200~12,000) substance was isolated from cultures of brown-rot basidiomycete Tyromyces palustris . It contained about 61% protein, 24% neutral carbohydrate, and 0.12% Fe(II) by weight. The glycopeptide containing Fe(II) alone reduced O 2 to · OH and catalyzed redox reactions between an electron donor, such as NADH, and O 2 to produce H 2 O 2 and to reduce H 2 O 2 to · OH. The glycopeptide reduced Fe(III) to Fe(II) (1.7 µ M/mg). The glycopeptide was a glycosylated peptide and had about 0.83 µ M glycosylamine/mg.