Supercritical fluid chromatography using methacrylate‐based monolithic column for the separation of polar analytes

Abstract A poly( N , N ‐dimethyl‐ N ‐methacryloyloxyethyl‐ N ‐(3‐sulfopropyl) ammonium betaine‐ co ‐ethylene dimethacrylate) monolith was prepared in a 100 × 2.0 mm id stainless‐steel column and was investigated for supercritical fluid chromatography. Optimization of its porosity was performed by changing the conditions of polymerization. Then, the chemical group of this column was confirmed by Fourier transform infrared spectroscopy and elemental analysis. The morphology was characterized by scanning electron microscopy. The prepared column showed good repeatability based on the retention factor of adenine, uracil and cytosine to calculate their run‐to‐run, day‐to‐day, column‐to‐column, and batch‐to‐batch relative standard deviations. Those values were less than 1.9% ( n = 10), 6.5% ( n = 3), 5.6% ( n = 3), and 1.7% ( n = 3), respectively. In addition, the column was found to be stable within 3 and 10 days with relative standard deviations less than 6.5 and 8.5%, respectively. These results indicated that the columns showed good reproducibility and stability. Compared with liquid chromatogaphy, supercritical fluid chromatography provided better kinetic performance and selectivity. Finally, several neutral, acid, and basic polar analytes were utilized to test its application. The results demonstrated that the prepared column exhibited a good separation performance and showed great potential in supercritical fluid chromatography.