Zeta电位
固体脂质纳米粒
细胞毒性
粒径
脂质体
动力学
药物输送
纳米颗粒
生物物理学
活力测定
阿糖胞苷
材料科学
体外
化学
纳米技术
色谱法
生物化学
医学
生物
外科
化疗
量子力学
物理化学
物理
作者
Shelly Roselyn Van Eyssen,Doğa Kavaz
标识
DOI:10.1080/02652048.2021.1992028
摘要
To synthesise cytarabine-loaded SLNs modified with the RGD peptide as a ligand, suitable for effective cancer therapy.SLNs were synthesised by the high shear, hot homogenisation technique. A 2 level 3 factor analysis was used in optimisation. Particle size, zeta potential, poly-dispersion index and surface morphology were measured. Drug encapsulation, drug release, release kinetics, nanoparticle stability and chemical structure were determined. LIVE/DEAD® Fluorescence Assay was used to qualify cytotoxicity and Tryphan Blue assay to quantify.Cyt-SLNs exhibited a size of 161 ± 2.25 nm, a PDI of 0.49 ± 0.15 and a zeta potential of -19.8 mV. Entrapment fell at 88.87 ± 0.02% and release at 83.5 ± 0.95%. The in vitro release kinetics pointed towards a diffusion-based drug release mechanism. SLNs remained stable for 60 d. Cytotoxicity studies revealed that conjugation of the ligand with the RDG peptide resulted in a significant decrease in cell viability in both cell lines.Overall, the study suggests that RGD-SLN-cyt can be used for effective cancer therapy.
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