瘦素
内分泌学
内科学
受体
信使核糖核酸
星形胶质细胞
小鼠苗条素受体
生物
神经科学
医学
中枢神经系统
肥胖
基因
遗传学
作者
Hung Hsuchou,Weihong Pan,Maria J. Barnes,Abba J. Kastin
出处
期刊:Peptides
[Elsevier]
日期:2009-12-01
卷期号:30 (12): 2275-2280
被引量:63
标识
DOI:10.1016/j.peptides.2009.08.023
摘要
We recently reported that mouse astrocytes express leptin receptors (ObR), and that obesity induces upregulation of astrocytic ObR. To provide further evidence of the importance of astrocytic ObR expression, we performed double-labeling fluorescent in situ hybridization (FISH) and immunohistochemistry in the rat hypothalamus. Laser confocal microscopic image analysis showed that ObR mRNA was present in glial fibrillary acidic protein (+) cells that show distinctive astrocytic morphology as well as in neurons. In addition to the presence of ObR mRNA, ObR protein was shown in both astrocytes and neurons in the rat hypothalamus by double-labeling immunohistochemistry. In cultured rat C6 astrocytoma cells treated with different doses of lipopolysaccharide for 6h, the mRNA for ObRa or ObRb did not show significant changes, as measured by quantitative RT-PCR. However, the protein expression of both ObRa and ObRb, determined by Western blotting, was increased after the C6 cells were treated with either lipopolysaccharide or tumor necrosis factor-alpha. The results indicate that astrocytic ObR expression is present in rats as well as mice, and that it probably plays a role in the neuroinflammatory response.
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