超顺磁性
吸附
磁铁矿
材料科学
扫描电子显微镜
透射电子显微镜
水溶液中的金属离子
磁性纳米粒子
蛋白质吸附
化学工程
核化学
金属
色谱法
分析化学(期刊)
纳米技术
磁化
化学
纳米颗粒
复合材料
冶金
有机化学
磁场
物理
工程类
量子力学
作者
Min Zhang,Dan Cheng,Xi‐Wen He,Langxing Chen,Yukui Zhang
标识
DOI:10.1002/asia.200900463
摘要
Abstract Magnetic silica‐coated magnetite (Fe 3 O 4 ) sub‐microspheres with immobilized metal‐affinity ligands are prepared for protein adsorption. First, magnetite sub‐microspheres were synthesized by a hydrothermal method. Then silica was coated on the surface of Fe 3 O 4 particles using a sol–gel method to obtain magnetic silica sub‐microspheres with core‐shell morphology. Next, the trichloro(4‐chloromethylphenyl) silane was immobilized on them, reacted with iminodiacetic acid (IDA), and charged with Cu 2+ . The obtained magnetic silica sub‐microspheres with immobilized Cu 2+ were applied for the absorption of bovine hemoglobin (BHb) and the removal of BHb from bovine blood. The size, morphology, and magnetic properties of the resulting magnetic micro(nano) spheres were investigated by using scanning microscopy (SEM), transmission electron microscopy (TEM), X‐ray diffraction (XRD), and a vibrating sample magnetometer (VSM). The measurements showed that the magnetic sub‐microspheres are spherical in shape, very uniform in size with a core‐shell, and are almost superparamagnetic. The saturation magnetization of silica‐coated magnetite (Fe 3 O 4 ) sub‐microspheres reached about 33 emu g −1 . Protein adsorption results showed that the sub‐microspheres had a high adsorption capacity for BHb (418.6 mg g −1 ), low nonspecific adsorption, and good removal of BHb from bovine blood. This opens a novel route for future applications in removing abundant proteins in proteomic analysis.
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