Determination of the sesquiterpene dialdehyde polygodial by high‐pressure liquid chromatography

Abstract A high‐pressure liquid chromatographic procedure for the determination of the antifeedant polygodial in Polygonum hydropiper leaves was developed. For leaves, the method consists of a selective extraction with hexane: EtOAc (8:2), pre‐purification of the evaporated extract over a solid phase C2 column, separation with a C18 RP‐HPLC column and detection/quantitation by means of UV at 233 nm. As internal standard α‐ionone is used. Advantages of the method are its selectivity and speed. Various aspects such as extraction efficiency, recovery, peak purity and reproducibility have been evaluated. The minimal detectable quantity is 1 ng.