抗体
免疫球蛋白轻链
克隆(Java方法)
细胞培养
细胞融合
分子生物学
单克隆抗体
生物
多发性骨髓瘤
细胞
免疫球蛋白重链
免疫学
基因
遗传学
作者
John F. Kearney,Andreas Radbruch,Bernhard Liesegang,Klaus Rajewsky
出处
期刊:Journal of Immunology
[The American Association of Immunologists]
日期:1979-10-01
卷期号:123 (4): 1548-1550
被引量:1461
标识
DOI:10.4049/jimmunol.123.4.1548
摘要
Abstract We have isolated a subclone of the mouse myeloma cell line P3-X63-Ag8 that does not express immunoglobulin heavy or light chains. This clone X63-Ag8.653 can be used for efficient fusion with antibody-forming cells to obtain hybrid cell lines producing pure monoclonal antibodies. Screening of hybrid cell lines for specificity and immunoglobulin classes was done with a modified enzyme-linked immunosorbent assay.
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