平原的
再生(生物学)
生物
转录组
计算生物学
背景(考古学)
基因
诱导多能干细胞
细胞生物学
基因表达
遗传学
胚胎干细胞
古生物学
作者
Guanshen Cui,Kangning Dong,Jia‐Yi Zhou,Shang Li,Ying Wu,Qinghua Han,Bofei Yao,Qunlun Shen,Yongliang Zhao,Ying Yang,Jun Cai,Shihua Zhang,Yun‐Gui Yang
标识
DOI:10.1038/s41467-023-39016-0
摘要
Abstract Whole-body regeneration of planarians is a natural wonder but how it occurs remains elusive. It requires coordinated responses from each cell in the remaining tissue with spatial awareness to regenerate new cells and missing body parts. While previous studies identified new genes essential to regeneration, a more efficient screening approach that can identify regeneration-associated genes in the spatial context is needed. Here, we present a comprehensive three-dimensional spatiotemporal transcriptomic landscape of planarian regeneration. We describe a pluripotent neoblast subtype, and show that depletion of its marker gene makes planarians more susceptible to sub-lethal radiation. Furthermore, we identified spatial gene expression modules essential for tissue development. Functional analysis of hub genes in spatial modules, such as plk1 , shows their important roles in regeneration. Our three-dimensional transcriptomic atlas provides a powerful tool for deciphering regeneration and identifying homeostasis-related genes, and provides a publicly available online spatiotemporal analysis resource for planarian regeneration research.
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