脂肪酶
化学
吸附
溶剂
甲醇
傅里叶变换红外光谱
浸出(土壤学)
生物柴油
南极洲假丝酵母
离子液体
固定化酶
结晶度
色谱法
核化学
化学工程
有机化学
酶
催化作用
工程类
结晶学
土壤科学
土壤水分
环境科学
作者
Eyad Sowan,Hanifa Taher,MD. Mozumder,Sulaiman Al-Zuhair
标识
DOI:10.1016/j.enzmictec.2023.110243
摘要
The zeolitic imidazole framework (ZIF)- 8 was tested as a support to enhance the stability of immobilized lipase. The lipase immobilized on ZIF-8, through surface attachment and encapsulation, was used for the simultaneous cell disruption and oil extraction from untreated, wet microalgal paste. The successful attachment of the enzyme to ZIF-8 was confirmed via Fourier-transform infrared spectroscopy. The attachment of the enzyme did not significantly affect the crystallinity or morphology of ZIF-8 crystals. The encapsulated lipase@ZIF-8 system showed higher stability than the adsorbed system, due to its reduced vulnerability to leaching. After five cycles, the encapsulated lipase@ZIF-8 retained 32% of its initial activity, whereas, for the adsorbed lipase@ZIF-8, it reduced to 21%. An increase in methanol amount greater than 0.2 mL was shown to have a negative effect on enzyme activity. The fatty acid methyl ester yield increased significantly with an increase in the extraction- duration (up to 3 h), after which the effect faded until 5 h, after which the equilibrium yield was reached. Changing the composition of the thermoresponsive switchable solvent (TSS) showed that a higher FAME yield could be achieved by increasing the percentages of Ionic Liquid (IL) and polypropylene glycol and reducing the water percentage. Further studies are needed to optimize the TSS composition and its effects on the process.
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