Defect-Engineered Coordination Compound Nanoparticles Based on Prussian Blue Analogues for Surface-Enhanced Raman Spectroscopy

Surface-enhanced Raman spectroscopy (SERS) is a powerful tool for label-free chemical analysis. The emergence of nonmetallic materials as SERS substrates, offering chemical signal enhancements, presents an exciting direction for achieving reproducible and biocompatible SERS, a challenge with traditional metallic substrates. Despite the potential, the realm of nonmetallic SERS substrates, particularly nanoparticles, remains largely untapped. Here, we present defect-engineered coordination compounds (DECCs) based on Prussian blue analogues (PBAs) as a class of nonmetallic nanoparticle-based SERS substrates. We demonstrate the utility and flexibility of the DECC template by incorporating various metal (M) elements into PBAs to synthesize nanoparticles that deliver substantial chemical mechanism (CM)-based enhancements to the Raman signal with a ∼ 10 8 -fold increase. The introduction of the M-PBA-based DECC nanoparticles as a class of SERS substrates represents a pioneering stride, enabling the straightforward and systematic exploration of a library of compounds for SERS-based analysis of a wide range of target molecules, especially biomolecules.