Preparation, Characterization, and Drug Release of Electrospun Core‐Shell Structured Hyaluronic Acid‐Bovine Serum Albumin/Poly(ε‐Caprolactone) Nanofiber Membranes

ABSTRACT Albumin has broad applications in tissue engineering, yet challenges remain in maintaining activity, loading capacity, and controlled release. In this study, we employed electrospinning to fabricate protein‐polysaccharide/poly(ε‐caprolactone) (PCL) nanofibers with a core‐shell structure. High‐magnification projection microscopy characterized the spinnability of hyaluronic acid‐bovine serum albumin (HyA‐BSA) solution. Scanning electron microscopy and high‐magnification projection microscopy demonstrated that the nanofibers had uniform diameter and internal structure, with Fourier‐transform infrared spectroscopy confirming the successful encapsulation of HyA‐BSA within PCL fibers. X‐ray diffraction indicated that the crystallite size of PCL decreased with increasing Span80 and HyA‐BSA content. Tensile and water contact angle tests revealed improvements in the flexibility and wettability of the nanofibers. In vitro drug tests indicated that the BSA loading and release rate could be modulated by varying Span80 and HyA‐BSA concentrations, with a total release time exceeding 30 days. Overall, this research enables prolonged drug release and offers new insights for protein encapsulation.