反向重复
生物
转座因子
发起人
基因
水稻
遗传学
转录因子
植物
基因表达
基因组
作者
Hui Zhang,Juncheng Zhang,Pengkun Xu,Ming Li,Yibo Li
摘要
A class I PCF type, TCP4, was identified as a transcription factor associated with both grain size and tillering through a DNA pull down-MS assay combined with a genome-wide association study. This transcription factor was found to have a significant role in the variations among the 533 rice accessions, dividing them into two main subspecies. A Tourist-like miniature inverted-repeat transposable element (MITE) was discovered in the promoter of TCP4 in Japonica/geng accessions (TCP4M+), which was found to suppress the expression of TCP4 at transcriptional level. The MITE deleted haplotype (TCP4M-) was mainly found in Indica/xian accessions. ChIP-qPCR and EMSA assays demonstrated the binding of TCP4 to promoters of grain reservoir genes such as SSIIa and Amy3D in vivo and in vitro, respectively. The introduction of the genomic sequence of TCP4M+ into different TCP4M- cultivars was found to affect the expression of TCP4 in the transgenic rice, resulting in decreased expression of its downstream target gene SSIIa, increased tiller number, and decreased seed length. This study revealed that a Tourist-like MITE contributes to subspecies divergence by regulating the expression of TCP4 in response to environmental pressure, thus balancing the division of source and sink by regulating the starch biosynthesis in rice.
科研通智能强力驱动
Strongly Powered by AbleSci AI