沃尔巴克氏菌
生物
盲蝽
迪罗菲拉里亚
遗传多样性
丝虫病
线虫
16S核糖体RNA
念珠菌
系统发育树
动物
遗传学
寄主(生物学)
蠕虫
基因
生态学
人口
人口学
社会学
作者
Patchana Kamkong,Wanarit Jitsamai,Kritsada Thongmeesee,Panan Ratthawongjirakul,Piyanan Taweethavonsawat
出处
期刊:Acta Tropica
[Elsevier]
日期:2023-10-01
卷期号:246: 107000-107000
标识
DOI:10.1016/j.actatropica.2023.107000
摘要
Canine filariasis is caused by nematodes from the family Onchocercidae, which is transmitted by arthropod vectors. The disease is commonly found in Southeast Asia and exists worldwide. Some filarial nematodes are associated with intracellular bacteria of the genus Wolbachia, which plays an important role in embryogenesis, molting, and the long-term survival of adult worms. This study aims to characterize Wolbachia sp. and determine the association between Wolbachia and canine filarial nematode species in Thailand. A total of 46 dog blood samples that were naturally infected with filarial nematodes were obtained to identify filarial nematode species by Giemsa stained under a light microscope and confirmed using the molecular technique. In order to characterize Wolbachia sp., the nested PCR assay targeting the 16S rRNA gene showed that all samples of Dirofilaria immitis and fifteen samples of Candidatus Dirofilaria hongkongensis were grouped into Wolbachia supergroup C. In addition, all samples of Brugia spp. and five samples of Candidatus Dirofilaria hongkongensis were classified into Wolbachia supergroup D. The genetic diversity analysis conducted using the 16S rRNA gene revealed a similar result when analyzed through phylogenetic tree analysis. This is the first genetic diversity study of Wolbachia of Candidatus Dirofilaria hongkongensis in infected dogs in Thailand.
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