Effects of thermal exposure to disposable plastic tableware on human gut microbiota and metabolites: A quasi-experimental study

The aim of this quasi-experimental study was to determine the alterations in gut microbiota and metabolism in humans who consume hot food served in disposable plastic tableware (DPT). Participants in the exposure and control groups were provided three hot meals in DPT (n = 30) or non-DPT (n = 30), respectively. After a month of observation, individuals in the exposure group discontinued the three meals provided in DPT (n = 27) for 1 month as the post-exposure group. Fecal samples were collected and tested for microplastics (MPs) detection using LDIR and gut microbiota identification based on the 16 S rRNA. Urine samples were used for metabolite analysis using LC-MS/MS. Results showed that the level of MPs in feces was lower in the post-exposure group compared with the exposure group. Furthermore, the abundance of the phyla Actinobacteria, Proteobacteria, Firmicutes, and Bacteroidota in the exposure and post-exposure groups were significantly different compared with the control group. Changes in microbiota abundance and metabolite levels were mainly associated with central nervous system effects, energy metabolism, and inflammation, suggesting that thermal exposure to DPT for 1 month has considerable health effects. With the development of the takeaway food industry, disposable plastic tableware is widely used, causing pollution to the environment as well as potential health hazards to the population. The heat released of freshly prepared food accelerates the rate of release and leaching of hazardous substances from disposable plastic tableware, resulting in the health balance of people who consume takeaway food over a long period of time being more susceptible to hazards. It is important to make up for the lack of animal studies in the hazard evaluation of pollutants by directly observing the population thermally exposed to disposable plastic tableware.