The impact of serum-free culture on HEK293 cells: From the establishment of suspension and adherent serum-free adaptation cultures to the investigation of growth and metabolic profiles

悬浮培养 适应(眼睛) 悬挂(拓扑) 细胞培养 化学 生物 细胞生物学 遗传学 数学 神经科学 同伦 纯数学
作者
Mi‐Ae Jang,Ellen Sofie Pete,Per Bruheim
出处
期刊:Frontiers in Bioengineering and Biotechnology [Frontiers Media]
卷期号:10 被引量:23
标识
DOI:10.3389/fbioe.2022.964397
摘要

Serum-free cultures are preferred for application in clinical cell therapy and facilitate the purification processes of bioproducts, such as vaccines and recombinant proteins. It can replace traditional cell culture - eliminating potential issues posed by animal-derived serum supplementation, such as lot to lot variation and risks of pathogen infection from the host animal. However, adapting cells to serum-free conditions can be challenging and time-consuming, and is cell line and medium dependent. In addition, the knowledge of the impact of serum-free culture on cellular metabolism is limited. Herein, we successfully established serum-free suspension and adherent cultures through two adaptation procedures for HEK293 cells in serum-free Freestyle 293 medium. Furthermore, growth kinetics and intracellular metabolic profiles related to central carbon metabolism were investigated. The entire adaptation procedure took 1 month, and high cell viability (>90%) was maintained throughout. The serum-free adherent culture showed the best growth performance, measured as the highest cell density and growth rate. The largest differences in metabolic profiles were observed between culture modes (adherent vs. suspension), followed by culture medium condition (control growth medium vs. serum-free medium). Metabolic differences related to the adaptation procedures were only seen in suspension cultures. Interestingly, the intracellular itaconate concentration was significantly higher in suspension cells compared to adherent cells. Furthermore, when the cells back-adapted from serum-free to serum-supplemented control medium, their metabolic profiles were immediately reversed, highlighting the effect of extracellular components on metabolic phenotype. This study provides strategies for efficient serum-free cultivation and deeper insights into the cellular responses related to growth and metabolism responses to diverse culture conditions.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
雪流星完成签到 ,获得积分10
刚刚
祖飞扬发布了新的文献求助10
1秒前
1秒前
Hello应助xinxinxin91采纳,获得10
2秒前
香蕉觅云应助桃李采纳,获得10
2秒前
杂货铺老板娘完成签到,获得积分10
2秒前
Coco完成签到,获得积分10
2秒前
LongSun完成签到,获得积分10
3秒前
酒辞完成签到,获得积分10
4秒前
阿强完成签到,获得积分10
4秒前
4秒前
王正浩完成签到 ,获得积分10
4秒前
豆子完成签到,获得积分10
4秒前
尚白swqd发布了新的文献求助10
5秒前
5秒前
5秒前
Dali完成签到,获得积分10
6秒前
NexusExplorer应助zzznznnn采纳,获得10
6秒前
所所应助Jsihao采纳,获得10
6秒前
6秒前
聂亦发布了新的文献求助10
7秒前
7秒前
科研通AI6应助xldhts采纳,获得10
7秒前
7秒前
的的的维尔完成签到,获得积分20
8秒前
猴猴完成签到,获得积分10
8秒前
科研通AI6应助淡定的天问采纳,获得10
8秒前
麻薯完成签到,获得积分10
9秒前
阿文在读研完成签到,获得积分10
9秒前
9秒前
pansy发布了新的文献求助10
9秒前
斯文败类应助祖飞扬采纳,获得10
10秒前
南瓜完成签到,获得积分10
10秒前
沉静忆安完成签到 ,获得积分10
10秒前
ynlqjqx发布了新的文献求助10
11秒前
斯文败类应助shengdong采纳,获得10
11秒前
bkagyin应助wang采纳,获得10
11秒前
可一可再完成签到 ,获得积分10
11秒前
猴猴发布了新的文献求助10
12秒前
12秒前
高分求助中
(应助此贴封号)【重要!!请各用户(尤其是新用户)详细阅读】【科研通的精品贴汇总】 10000
Einführung in die Rechtsphilosophie und Rechtstheorie der Gegenwart 1500
Cowries - A Guide to the Gastropod Family Cypraeidae 1200
青少年心理适应性量表(APAS)使用手册 700
Socialization In The Context Of The Family: Parent-Child Interaction 600
“Now I Have My Own Key”: The Impact of Housing Stability on Recovery and Recidivism Reduction Using a Recovery Capital Framework 500
The Red Peril Explained: Every Man, Woman & Child Affected 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 内科学 生物化学 物理 计算机科学 纳米技术 遗传学 基因 复合材料 化学工程 物理化学 病理 催化作用 免疫学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 5011114
求助须知:如何正确求助?哪些是违规求助? 4252631
关于积分的说明 13251882
捐赠科研通 4055123
什么是DOI,文献DOI怎么找? 2218038
邀请新用户注册赠送积分活动 1227685
关于科研通互助平台的介绍 1149619