化学
生物降解
矿化(土壤科学)
新陈代谢
氮气循环
氮气
营养物
微生物
磺胺甲恶唑
降级(电信)
生物化学
代谢中间体
食品科学
代谢途径
环境化学
抗生素
有机化学
基因
电信
16S核糖体RNA
计算机科学
作者
Yuzhe He,Lei Liu,Qi Wang,Xiaoqi Dong,Jingfei Huang,Xiaoshan Jia,Xingxing Peng
标识
DOI:10.1016/j.jhazmat.2024.133558
摘要
Sulfamethoxazole (SMX) is widely employed as an antibiotic, while its residue in environment has become a common public concern. Using 100 mg/L SMX as the sole nutrient source, the acclimated sludge obtained by this study displayed an excellent SMX degradation performance. The addition of SMX resulted in significant microbiological differentiation within the acclimated sludge. Microbacterium (6.6%) was identified as the relatively dominant genera in metabolism group that used SMX as sole carbon source. Highly expressed proteins from this strain strongly suggested its essential role in SMX degradation, while the degradation of SMX by other strains (Thaurea 78%) in co-metabolism group appeared to also rely on this strain. The interactions of differentially expressed proteins were primarily involved in metabolic pathways including TCA cycle and nitrogen metabolism. It is concluded that the sulfonamides might serve not only as the carbon source but also as the nitrogen source in the reactor. A total of 24 intermediates were identified, 13 intermediates were newly reported. The constructed pathway suggested the mineralizing and nitrogen conversion ability towards SMX. Batch experiments also proved that the acclimated sludge displayed ability to biodegrade other sulfonamides, including SM2 and SDZ and SMX-N could be removed completely.
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