基因组
生物
单倍型
生物合成
计算生物学
遗传学
基因
基因型
作者
Bao Nie,Xueqing Chen,Zhuangwei Hou,Miaoxian Guo,Cheng Li,Wei Sun,Jiaojiao Ji,Lanlan Zang,S. L. Yang,Pengxiang Fan,Wenhao Zhang,Hang Li,Yuzhu Tan,Wei Li,Li Wang
出处
期刊:Science Advances
[American Association for the Advancement of Science (AAAS)]
日期:2024-02-09
卷期号:10 (6)
被引量:1
标识
DOI:10.1126/sciadv.adj6547
摘要
Butylphthalide is one of the first-line drugs for ischemic stroke therapy, while no biosynthetic enzyme for butylphthalide has been reported. Here, we present a haplotype-resolved genome of Ligusticum chuanxiong , a long-cultivated and phthalide-rich medicinal plant in Apiaceae. On the basis of comprehensive screening, four Fe(II)- and 2-oxoglutarate–dependent dioxygenases and two CYPs were mined and further biochemically verified as phthalide C-4/C-5 desaturases (P4,5Ds) that effectively promoted the forming of ( S )-3- n -butylphthalide and butylidenephthalide. The substrate promiscuity and functional redundancy featured for P4,5Ds may contribute to the high phthalide diversity in L. chuanxiong . Notably, comparative genomic evidence supported L. chuanxiong as a homoploid hybrid with Ligusticum sinense as a potential parent. The two haplotypes demonstrated exceptional structure variance and diverged around 3.42 million years ago. Our study is an icebreaker for the dissection of phthalide biosynthetic pathway and reveals the hybrid origin of L. chuanxiong , which will facilitate the metabolic engineering for ( S )-3- n -butylphthalide production and breeding for L. chuanxiong .
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