荧光
光漂白
单体
二聚体
荧光蛋白
化学
衍生工具(金融)
绿色荧光蛋白
光漂白后的荧光恢复
黄色荧光蛋白
生物物理学
亮度
光化学
生物化学
生物
聚合物
有机化学
光学
物理
金融经济学
基因
经济
作者
Esther Ivorra-Molla,Dipayan Akhuli,Martin B. L. McAndrew,William A. Scott,Lokesh Kumar,Saravanan Palani,Masanori Mishima,A. Crow,Mohan K. Balasubramanian
标识
DOI:10.1038/s41587-023-02018-w
摘要
Abstract StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. Despite favorable fluorescence properties, use of StayGold as a fluorescent tag is limited because it forms a natural dimer. Here we report the 1.6 Å structure of StayGold and generate a derivative, mStayGold, that retains the brightness and photostability of the original protein while being fully monomeric.
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