体细胞核移植
重编程
生物
克隆(编程)
DNA甲基化
基因组印记
体细胞
印记(心理学)
滋养层
胚胎
遗传学
细胞生物学
胚泡
细胞
胚胎发生
基因
胎儿
胎盘
怀孕
基因表达
计算机科学
程序设计语言
作者
Zhaodi Liao,J Zhang,Shiyu Sun,Yunwei Li,Yuting Xu,Chunyang Li,Ji Cao,Yanhong Nie,Zhenmin Niu,Jingwen Li,Falong Lu,Zhen Liu,Qiang Sun
标识
DOI:10.1038/s41467-023-43985-7
摘要
Abstract Somatic cell nuclear transfer (SCNT) successfully clones cynomolgus monkeys, but the efficiency remains low due to a limited understanding of the reprogramming mechanism. Notably, no rhesus monkey has been cloned through SCNT so far. Our study conducts a comparative analysis of multi-omics datasets, comparing embryos resulting from intracytoplasmic sperm injection (ICSI) with those from SCNT. Our findings reveal a widespread decrease in DNA methylation and the loss of imprinting in maternally imprinted genes within SCNT monkey blastocysts. This loss of imprinting persists in SCNT embryos cultured in-vitro until E17 and in full-term SCNT placentas. Additionally, histological examination of SCNT placentas shows noticeable hyperplasia and calcification. To address these defects, we develop a trophoblast replacement method, ultimately leading to the successful cloning of a healthy male rhesus monkey. These discoveries provide valuable insights into the reprogramming mechanism of monkey SCNT and introduce a promising strategy for primate cloning.
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