MPTP公司
多巴胺能
多巴胺
氧化应激
医学
帕金森病
酪氨酸羟化酶
药理学
3,4-二羟基苯乙酸
左旋多巴
神经毒素
内科学
内分泌学
高香草酸
疾病
血清素
受体
作者
Jun‐Ho Song,Lian Liu,Zhiquan Li,Ting Mei,Jianfei Zhang,Lei Zhou,Xin Chen,Yunzhu Shang,Tao Sun,Yan Luo,Yu Jiang,Tan D,Xiaoling Tong,Fangyin Dai
出处
期刊:BMC Medicine
[Springer Nature]
日期:2022-10-28
卷期号:20 (1)
被引量:7
标识
DOI:10.1186/s12916-022-02621-9
摘要
Abstract Background Parkinson’s disease (PD) is the second most common neurodegenerative disease in middle-aged and elderly populations, whereas there is no cure for PD so far. Novel animal models and medications await development to elucidate the aetiology of PD and attenuate the symptoms, respectively. Methods A neurotoxin, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), was used in the current study to establish a PD pathologic model in silkworms. The time required to complete specific behaviours was recorded. Dopamine content was detected by ultra-performance liquid chromatography (UPLC). The activity of insect tyrosine hydroxylase (TH) was determined using a double-antibody sandwich method. Oxidative stress was assessed by changes in antioxidant enzyme activity and the content of oxidative products. Results MPTP-treated silkworms were characterized by impaired motor ability, reduced dopamine content, and elevated oxidative stress level. The expression of TH, a dopamine biosynthetic enzyme within dopaminergic neurons in the brain, was significantly reduced, indicating that dopaminergic neurons were damaged. Moreover, MPTP-induced motility impairment and reduced dopamine level in the silkworm PD model could be rescued after feeding a combination of levodopa (L-dopa [LD]) and carbidopa (CD). MPTP-induced oxidative damage was also alleviated, in ways consistent with other PD animal models. Interestingly, administration of Lycium barbarum polysaccharide (LBP) improved the motor ability, dopamine level, and TH activity, and the oxidative damage was concomitantly reduced in the silkworm PD model. Conclusions This study provides a promising animal model for elucidating the pathogenesis of PD, as well as a relevant preliminary drug screening (e.g., LBP) and evaluation.
科研通智能强力驱动
Strongly Powered by AbleSci AI