Molecular and functional characterization of an antenna‐enriched glutathione S‐transferase, LoryGSTd1, with cinnamaldehyde and cis‐jasmone degradation ability in the Lissorhoptrus oryzophilus

Abstract BACKGROUND Glutathione S‐transferases (GSTs) play a vital role in maintaining olfactory sensitivity. However, their roles in odorant inactivation in the rice water weevil (RWW, Lissorhoptrus oryzophilus ) remain largely unknown. RESULTS Through antennal transcriptome analysis of the RWW, this study identified 20 LoryGSTs. Quantitative reverse transcription polymerase chain reaction (qRT‐PCR) revealed high expression of LoryGSTd1 in antennae. Liquid chromatography–mass spectrometry (LC–MS) showed that recombinant LoryGSTd1 efficiently degraded cinnamaldehyde and cis ‐jasmone, with their metabolites identified. Molecular docking analysis further identified ARG68, LEU99, and ILE103 as critical binding residues in LoryGSTd1. Two‐choice behavioral assays and electroantennogram (EAG) recordings demonstrated that RWW exhibited significant responses to LoryGSTd1‐associated odorants. CONCLUSION Our findings suggest that LoryGSTd1 participates in odorant metabolism, implying its potential as a molecular target for developing innovative pest control strategies. © 2025 Society of Chemical Industry.