免疫沉淀
色谱法
串联质谱法
化学
液相色谱-质谱法
串联
Copeptin蛋白
质谱法
材料科学
医学
生物化学
内科学
基因
复合材料
加压素
作者
Danni Mu,Xiaoli Ma,Yichen Ma,Danchen Wang,Yutong Zou,Ying Zhu,Jian Zhong,Dandan Sun,Yicong Yin,Yumeng Gao,Yuemeng Li,Shi Chen,Huijuan Zhu,Songlin Yu,Ling Qiu,Xinqi Cheng
标识
DOI:10.1515/cclm-2025-0022
摘要
OBJECTIVES: Copeptin, a stable and simple-to-measure surrogate marker for arginine vasopressin (AVP), demonstrates excellent clinical values, particularly in diagnosing polyuria-polydipsia syndromes. However, conventional immunoassay methods are limited and lack comparability. Our aim was to establish a targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying copeptin in human serum. METHODS: Copeptin was extracted from serum using immunoprecipitation, digested with trypsin, and prepared using anion exchange solid-phase extraction before LC-MS/MS detection. The analytical performance was validated in accordance with current guidelines. The method was compared to an immunofluorescent assay on the B.R.A.H.M.S platform. RESULTS: The LC-MS/MS method had a runtime of 8.5 min. The within-run precision ranged from 5.2 to 12.1 %, and total coefficients of variation ranged from 8.1 to 13.5 %. Copeptin quantitation showed linearity within the range of 5-1,000 pg/mL, with a limit of detection of 2.5 pg/mL. Recovery rates ranged from 95.2 to 103.1 %, and no significant matrix effect was observed with internal standard correction. The LC-MS/MS method had a good consistency with the immunoassay (r=0.926, slope=0.989). The reference interval for healthy individuals was 3.66-58.25 pg/mL. CONCLUSIONS: We demonstrated the accuracy and reliability of this targeted LC-MS/MS method for quantifying copeptin. This innovative application showed satisfactory precision, a wide linear range, and a low limit of detection. Clinical studies are anticipated to be conducted to assess diagnostic accuracy using this method.
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