Intranasal immunization with outer membrane vesicles (OMV) protects against airway colonization and systemic infection with Acinetobacter baumannii

鲍曼不动杆菌 免疫 微生物学 鼻腔给药 免疫 肺炎 医学 接种疫苗 免疫学 病毒学 生物 抗体 免疫系统 细菌 铜绿假单胞菌 内科学 遗传学
作者
Sophie L. Higham,Stephen Baker,Katie E. Flight,Aishwarya Krishna,Paul Kellam,Stephen T. Reece,John S. Tregoning
出处
期刊:Journal of Infection [Elsevier BV]
卷期号:86 (6): 563-573 被引量:25
标识
DOI:10.1016/j.jinf.2023.02.035
摘要

The multidrug-resistant bacteria Acinetobacter baumannii is a major cause of hospital-associated infection; a vaccine could significantly reduce this burden. The aim was to develop a clinically relevant model of A. baumannii respiratory tract infection and to test the impact of different immunization routes on protective immunity provided by an outer membrane vesicle (OMV) vaccine.BALB/c mice were intranasally challenged with isolates of oxa23-positive global clone GC2 A. baumannii from the lungs of patients with ventilator-associated pneumonia. Mice were immunized with OMVs by the intramuscular, subcutaneous or intranasal routes; protection was determined by measuring local and systemic bacterial load.Infection with A. baumannii clinical isolates led to a more disseminated infection than the prototype A. baumannii strain ATCC17978; with bacteria detectable in upper and lower airways and the spleen. Intramuscular immunization induced an antibody response but did not protect against bacterial infection. However, intranasal immunization significantly reduced airway colonization and prevented systemic bacterial dissemination.Use of clinically relevant isolates of A. baumannii provides stringent model for vaccine development. Intranasal immunization with OMVs was an effective route for providing protection, demonstrating that local immunity is important in preventing A. baumannii infection.
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