蔗渣
化学
发酵
乙醇燃料
纤维素乙醇
糠醛
柄毕赤酵母
水解物
生物燃料
食品科学
水解
乙醇发酵
醋酸
酶水解
乙醇
生物化学
生物技术
纤维素
生物
催化作用
作者
None Hemansi,Jitendra Kumar Saini
标识
DOI:10.1016/j.biortech.2023.128671
摘要
This study reports high gravity fed-batch simultaneous saccharification and fermentation (FB-SSF) of sequentially pretreated sugarcane bagasse (SCB) for enhanced bioethanol by employing multiple inhibitor tolerant Kluyveromyces marxianusJKH5 C60. FB-SSF with intermittent feeding of SCB (total 20 % solid loading) and enzyme (total dose of 20 FPU/g) at 6 and 12 h resulted in superior bioethanol production at42 °C. Under optimizedlab-scaleFB-SSF, the maximum ethanoltiter, efficiency and productivities were73.4 ± 1.2 g/L,78 % and 3.0 g/L/h, respectively, after 72 h in presence of inhibitors (acetic acid, furfural, and vanillin at 3, 1, and 1 g/L, respectively). Furthermore, pentose rich dilute acid hydrolysate of SCB was subjected to fermentation by Pichia stipitis NCIM 3499, resulting in ethanol titer of 6.8 g/L. Overall ethanol yield during the developed process was 260.1 g/kg native SCB, which proves industrial potential of the developed bioethanol conversion process.
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