RNA干扰
生物
小RNA
免疫
昆虫
植物免疫
核糖核酸
RNA沉默
遗传学
病毒学
免疫系统
基因
生态学
拟南芥
突变体
作者
Zelong Zhang,Xiaojing Wang,Jiafeng Lü,Haibin Lu,Zhuang-Xin Ye,Zhongtian Xu,Chaofeng Zhang,Jianping Chen,Junmin Li,Chuan‐Xi Zhang,Hai‐Jian Huang
标识
DOI:10.1073/pnas.2318783121
摘要
Communication between insects and plants relies on the exchange of bioactive molecules that traverse the species interface. Although proteinic effectors have been extensively studied, our knowledge of other molecules involved in this process remains limited. In this study, we investigate the role of salivary microRNAs (miRNAs) from the rice planthopper Nilaparvata lugens in suppressing plant immunity. A total of three miRNAs were confirmed to be secreted into host plants during insect feeding. Notably, the sequence-conserved miR-7-5P is specifically expressed in the salivary glands of N. lugens and is secreted into saliva, distinguishing it significantly from homologues found in other insects. Silencing miR-7-5P negatively affects N. lugens feeding on rice plants, but not on artificial diets. The impaired feeding performance of miR-7-5P-silenced insects can be rescued by transgenic plants overexpressing miR-7-5P. Through target prediction and experimental testing, we demonstrate that miR-7-5P targets multiple plant genes, including the immune-associated bZIP transcription factor 43 (OsbZIP43). Infestation of rice plants by miR-7-5P-silenced insects leads to the increased expression of OsbZIP43 , while the presence of miR-7-5P counteracts this upregulation effect. Furthermore, overexpressing OsbZIP43 confers plant resistance against insects which can be subverted by miR-7-5P. Our findings suggest a mechanism by which herbivorous insects have evolved salivary miRNAs to suppress plant immunity, expanding our understanding of cross-kingdom RNA interference between interacting organisms.
科研通智能强力驱动
Strongly Powered by AbleSci AI