化学
离子
离子阱
质谱法
四极离子阱
单细胞分析
丰度(生态学)
分析化学(期刊)
灵敏度(控制系统)
色谱法
细胞
生物化学
工程类
生物
有机化学
渔业
电子工程
作者
Xingyu Si,Xin Xiong,Sichun Zhang,Xiang Fang,Xinrong Zhang
标识
DOI:10.1021/acs.analchem.6b03390
摘要
Low-abundance metabolites or proteins in single-cell samples are usually undetectable by mass spectrometry (MS) due to the limited amount of substances in single cells. This limitation inspired us to further enhance the sensitivity of commercial mass spectrometers. Herein, we developed a technique named repeated ion accumulation by ion trap MS, which is capable of enhancing the sensitivity by selectively and repeatedly accumulating ions in a linear ion trap for up to 25 cycles. The increase in MS sensitivity was positively correlated with the number of repeated cycles. When ions were repeatedly accumulated for 25 cycles, the sensitivity of adenosine triphosphate detection was increased by 22-fold within 1.8 s. Our technique could stably detect low-abundance ions, especially MSn ions, at the single-cell level, such as 5-methylcytosine hydrolyzed from sample equivalent to ∼0.2 MCF7 cell. The strategy presented in this study offers the possibility to aid single-cell analysis by enhancing MS detection sensitivity.
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