过氧化氢
活性氧
纳米颗粒
氧化铁纳米粒子
羟基自由基
超氧化物
材料科学
生物物理学
化学
光化学
纳米技术
生物化学
激进的
酶
生物
作者
Anastasia K. Hauser,Mihail I. Mitov,Emily F. Daley,Ronald C. McGarry,Kimberly W. Anderson,J. Zach Hilt
出处
期刊:Biomaterials
[Elsevier]
日期:2016-10-01
卷期号:105: 127-135
被引量:155
标识
DOI:10.1016/j.biomaterials.2016.07.032
摘要
To increase the efficacy of radiation, iron oxide nanoparticles can be utilized for their ability to produce reactive oxygen species (ROS). Radiation therapy promotes leakage of electrons from the electron transport chain and leads to an increase in mitochondrial production of the superoxide anion which is converted to hydrogen peroxide by superoxide dismutase. Iron oxide nanoparticles can then catalyze the reaction from hydrogen peroxide to the highly reactive hydroxyl radical. Therefore, the overall aim of this project was to utilize iron oxide nanoparticles conjugated to a cell penetrating peptide, TAT, to escape lysosomal encapsulation after internalization by cancer cells and catalyze hydroxyl radical formation. It was determined that TAT functionalized iron oxide nanoparticles and uncoated iron oxide nanoparticles resulted in permeabilization of the lysosomal membranes. Additionally, mitochondrial integrity was compromised when A549 cells were treated with both TAT-functionalized nanoparticles and radiation. Pre-treatment with TAT-functionalized nanoparticles also significantly increased the ROS generation associated with radiation. A long term viability study showed that TAT-functionalized nanoparticles combined with radiation resulted in a synergistic combination treatment. This is likely due to the TAT-functionalized nanoparticles sensitizing the cells to subsequent radiation therapy, because the nanoparticles alone did not result in significant toxicities.
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