色谱法
肉毒神经毒素
分析物
生物分析
免疫分析
组合化学
作者
A. V. Orlov,S.L. Znoyko,V. R. Cherkasov,Maxim P. Nikitin,Petr I. Nikitin
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2016-10-17
卷期号:88 (21): 10419-10426
被引量:59
标识
DOI:10.1021/acs.analchem.6b02066
摘要
We present a multiplex quantitative lateral flow (LF) assay for simultaneous on-site detection of botulinum neurotoxin (BoNT) types A, B, and E in complex matrixes, which is innovative by virtually no sacrifice in performance while transition from the single-plex assays and by characteristics on the level of laboratory quantitative methods. The novel approach to easy multiplexing is realized via joining an on-demand set of single-plex LF strips, which employ magnetic nanolabels, into a miniature cylinder cartridge that mimics LF strip during all assay stages. The cartridge is read out by an original portable multichannel reader based on the magnetic particle quantification technique. The developed reader offers the unmatched 60 zmol detection limit and 7-order linear dynamic range for volumetric registration of magnetic labels inside a cartridge of several millimeters in diameter regardless of its optical transparency. Each of the test strips, developed here as building blocks for the multiplex assay, can...
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