发光
生物传感器
检出限
线性范围
材料科学
核酸
纳米技术
相(物质)
胶体金
化学
纳米颗粒
光电子学
色谱法
生物化学
有机化学
作者
Xiaorong Liu,Chaonan He,Qi Huang,Mengmeng Yu,Qixin Zhuang,Haoxin Cheng,Yifei Yang,Xian Hao,Xiaolei Wang
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2022-01-01
卷期号:147 (11): 2378-2387
被引量:4
摘要
The development of portable solid-phase biosensors is of great significance for point-of-care testing (POCT). In this work, we constructed a simple visualized solid-phase biosensor based on luminescence resonance energy transfer (LRET) from upconversion nanoparticles (UCNPs) to gold nanoparticles (AuNPs) for quantitative detection of virus-specific nucleic acid sequences. The detection data showed that there was a linear relationship between the luminescence recovery of UCNPs and the concentrations of the target within the range of 5-100 nM, and the limit of detection (LOD) was 0.326 nM. Additionally, the luminescence recovery of UCNPs was visualized and quantitatively analyzed using a home-built luminescence image capture device and an open-source ImageJ software that can analyze and process images. Compared with conventional liquid-phase biosensors, the solid-phase analysis method we constructed not only has advantages in cost, portability and stability, but also is more conducive to the rapid acquisition and storage of the detected sample, which is expected to become a fast, efficient and reliable detection platform for POCT.
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