肝细胞癌
分子成像
原位
荧光寿命成像显微镜
临床前影像学
化疗
荧光
双光子激发显微术
医学
化学
病理
体内
癌症研究
内科学
生物
光学
生物技术
有机化学
物理
作者
Xiaofeng Wu,Rui Wang,Sujie Qi,Nahyun Kwon,Jingjing Han,Heejeong Kim,Haidong Li,Fabiao Yu,Juyoung Yoon
标识
DOI:10.1002/ange.202101190
摘要
Abstract Selective fluorescence imaging of biomarkers in vivo and in situ for evaluating orthotopic hepatocellular carcinoma (HCC) chemotherapy remains a great challenge due to current imaging agents suffering from the potential interferences of other hydrolases. Herein, we observed that carbamate unit showed a high selectivity toward the HCC‐related biomarker carboxylesterase (CE) for evaluation of treatment. A near‐infrared two‐photon fluorescent probe was developed to not only specially image CE activity in vivo and in situ but also target orthotopic liver tumor after systemic administration. The in vivo signals of the probe correlate well with tumor apoptosis, making it possible to evaluate the status of treatment. The probe enables the imaging of CE activity in situ with a high‐resolution three‐dimensional view for the first time. This study may promote advances in optical imaging approaches for precise imaging‐guided diagnosis of HCC in situ and its evaluation of treatment.
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