化学
碱基对
解旋酶
序列(生物学)
DNA纳米技术
分支迁移
DNA
组合化学
A-DNA
生物物理学
生物
霍利迪路口
DNA修复
生物化学
核糖核酸
基因
作者
Pepijn G. Moerman,Momčilo Gavrilov,Taekjip Ha,Rebecca Schulman
标识
DOI:10.1002/anie.202114581
摘要
The sequence-specific hybridization of DNA facilitates its use as a building block for designer nanoscale structures and reaction networks that perform computations. However, the strong binding energy of Watson-Crick base pairing that underlies this specificity also causes the DNA dehybridization rate to depend sensitively on sequence length and temperature. This strong dependency imposes stringent constraints on the design of multi-step DNA reactions. Here we show how an ATP-dependent helicase, Rep-X, can drive specific dehybridization reactions at rates independent of sequence length, removing the constraints of equilibrium on DNA hybridization and dehybridization. To illustrate how this new capacity can speed up designed DNA reaction networks, we show that Rep-X extends the range of conditions where the primer exchange reaction, which catalytically adds a domain provided by a hairpin template to a DNA substrate, proceeds rapidly.
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