Effects of Plant Hormones on Formation of Scopoletin and Scopolin in Tobacco Tissue Cultures

The addition of 2,4-dichlorophenoxyacetic acid (2,4-D), indoleacetic acid (IAA) and benzylaminopurine (BAP) increased the quantity of scopoletin and scopolin during the exponential growth phase. 2,4-D greatly increased the quantity of scopolin in cells. Naphthaleneacetic acid (NAA) and kinetin caused the accumulation of scopoletin in the medium during non-growth phase. The combination of NAA and BAP was more effective for formation of scopoletin and scopolin than the use of either hormone alone. 2,4-D stimulated the incorporation of scopoletin from the culture medium into the cells and the conversion of scopoletin to scopolin. The stimulatory action of 2,4-D was inhibited by KCN, but not by cycloheximide.