超氧化物歧化酶
丙二醛
活性氧
聚乙烯醇
谷胱甘肽过氧化物酶
微生物学
壳聚糖
白细胞介素
肿瘤坏死因子α
化学
细胞毒性
伤口愈合
羟脯氨酸
戊二醛
药理学
免疫学
体外
医学
氧化应激
细胞因子
生物化学
生物
色谱法
有机化学
作者
Sharareh Ahmadi,Mohammad Reza Farahpour,Zohreh Ghazi Tabatabaei
标识
DOI:10.1016/j.jddst.2022.103589
摘要
Chitosan (Csn) and polyvinyl alcohol (PVA) have mechanical strength properties and can be encapsulated with citral (Cil) into nanoemulsions (NEs) and applied for the treatment of infected wounds. This study investigates the effects PVA/Csn/Cil-NEs for the treatment of infected wounds in a mouse model. The NEs were prepared by the help of Cil, PVA and 10% and 20% Csn. In vitro release and antibacterial properties and also cytotoxicity analysis were done. The therapeutic formulations were prepared and administrated in circular wounds infected with Acinetobacter baumannii and Streptococcus pyogenes. Wound contraction, histopathological parameters total bacterial count, the serum concentrations of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), transforming growth factor-β or (TGF-β), interleukin-10 (IL-10), reactive oxygen species (ROS), and malondialdehyde (MDA), and activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD) were evaluated. The NEs were safe and addition of Csn decreased rapid release of Cil. The mice treated with the NEs containing higher concentrations of Csn exhibited lower bacterial count, the serum concentrations of TNF-α, IL-1β, ROS, MDA, edema, and immune cells and higher the serum concentrations of TGF-β and IL-10, activities SOD and GPx, and collagen deposition. In conclusion, the PVA/Csn/Cil-NEs expedited infected wounds in a mice model.
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