组织蛋白酶B
生物医学中的光声成像
分子成像
微气泡
细胞内
组织蛋白酶
癌症研究
体内
化学
医学
超声波
生物化学
放射科
生物
酶
光学
物理
生物技术
作者
Chenchen Wang,Wei Du,Chenfan Wu,Shan Dan,Miao Sun,Tong Zhang,Bin Wang,Yue Yuan,Gaolin Liang
标识
DOI:10.1002/anie.202114766
摘要
Cathepsin B (CTSB) is a lysosomal protease that is overexpressed in the early stage of many cancer types. Precise evaluation of CTSB expression in vivo may provide a promising method for the early diagnosis of cancers. By virtue of the high-resolution PA imaging modality, a "smart" photoacoustic (PA) probe Cypate-CBT, which can self-assemble to cypate-containing nanoparticles in response to abundant GSH and CTSB inside tumor cells, was developed for the sensitive and specific detection of CTSB activity. Compared with unmodified Cypate, our probe Cypate-CBT exhibited a 4.9-fold or 4.7-fold PA signal enhancement in CTSB-overexpressing MDA-MB-231 cancer cells or tumors, respectively, revealing intracellular accumulation of the probe after CTSB-initiated self-assembly. We expect Cypate-CBT to be employed as an effective PA imaging agent for clinical diagnosis of cancer at early stages.
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