苯酚
辣根过氧化物酶
戊二醛
葡萄糖氧化酶
水溶液
化学
固定化酶
化学工程
生物催化
介孔材料
核化学
色谱法
有机化学
催化作用
酶
反应机理
工程类
作者
Mohammad Razzaghi,Afzal Karimi,Zahra Ansari,Hassan Aghdasinia
标识
DOI:10.1016/j.jtice.2018.03.040
摘要
In this study, horseradish peroxidase (HRP) and glucose oxidase (GOx) were immobilized on mesoporous ZSM-5 nanoparticles by using glutaraldehyde as cross linking agent, and the prepared biocatalyst was characterized using SEM and EDAX mapping analysis. The resulted HRP/GOx/ZSM-5 biocatalyst was used for phenol removal from aqueous solution. In order to prevent the deactivation of HRP in the presence of excess H2O2, required H2O2 was produced by GOx in situ to activate HRP, which led to an increase in removal efficiency of phenol about 30%. Investigations on the removal efficiency of phenol for both immobilized and free enzymes indicated that immobilized enzymes have higher activity and less sensitivity to pH variation compared with free ones. In addition, the effect of parameters such as temperature, pH, HRP/GOx ratio, phenol and glucose concentration on the removal efficiency of phenol was investigated. Finally, an artificial neural network (ANN) was developed to model and express the relationship between removal efficiency of phenol and aforementioned parameters. The optimized values of parameters were determined by optimizing the resulted ANN model using genetic algorithm (GA).
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