小泡
细菌外膜
膜
化学
分离(微生物学)
细菌
脂泡
生物
生物物理学
细胞生物学
生物化学
色谱法
微生物学
大肠杆菌
遗传学
基因
作者
Paul Kohl,Franz G. Zingl,Thomas O. Eichmann,Stefan Schild
出处
期刊:Methods in molecular biology
日期:2018-01-01
卷期号:: 117-134
被引量:20
标识
DOI:10.1007/978-1-4939-8685-9_11
摘要
Outer membrane vesicles (OMVs) are naturally secreted from the bacterial cell surface and therefore localized in the cell-free supernatant of bacterial cultures. Here we describe methods for crude and density gradient-purified OMV isolation and protocols for control analyses for protein profiling (SDS-PAGE), detection of indicator proteins (immunoblot analysis), lipid profiling (lipid extraction and LC-MS analysis), vesicle size determination (NanoSight), rough estimation of biomass (TrayCell™), as well as quantifications of defined OMV components, e.g., proteins (Bradford) and LPS (Purpald).
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