Förster Resonance Energy Transfer‐Activated Processes in Smart Nanotheranostics Fabricated in a Sustainable Manner

Abstract Multilayer nanocarriers loaded with optically activated payloads are gaining increasing attention due to their anticipated crucial role for providing new mechanisms of energy transfers in the health‐oriented applications, as well as for energy storage and environmental protection. The combination of careful selection of optical components for efficient Förster resonance energy transfer, and surface engineering of the nanocarriers, allowed us to synthesize and characterize novel theranostic nanosystems for diagnosis and therapy of deep‐seated tumors. The cargo, constrained within the oil core of the nanocapsules, composed of NaYF 4 :Tm +3 , Yb +3 up‐converting nanoparticles together with a second‐generation porphyrin‐based photosensitizing agent—Verteporfin, assured requisite diagnostic and therapeutic functions under near‐IR laser excitation. The outer polyaminoacid shell of the nanocapsules was functionalized with a ligand—poly( l ‐glutamic acid) functionalized by PEG‐ylated folic acid—to ensure both a “stealth” effect and active targeting towards human breast cancer cells. The preparation criteria of all nanocarrier building blocks meet the requirements for sustainable and green chemistry practices. The multifunctionality of the proposed nanocarriers is a consequence of both the surface‐functionalized organic exterior part, which was accessible for selective accumulation in cancer cells, and the hydrophobic optically active interior, which shows phototoxicity upon irradiation within the first biological window.