东莨菪碱
青蒿素
化学
色谱法
高效液相色谱法
香豆素
药理学
医学
有机化学
免疫学
病理
替代医学
疟疾
恶性疟原虫
作者
Min Qing,Wei Lu,Manyuan Wang,Dong Zhang,Tianhang Zhou,Liang Li
出处
期刊:China journal of Chinese materia medica
[China Journal of Chinese Materia Medica]
日期:2014-09-01
被引量:1
标识
DOI:10.4268/cjcmm20141719
摘要
The objective of this study is to develop a sensitive and reliable high-performance liquid chromatography mass spectrometry (LC-MS) method for simultaneous determination of artemisinin, arteannuin B, artemisic acid, and scopoletin, and study the pharmacokinetics of the four constituents in mouse serum after oral administration of the four components to mice. The analytical column used was Agilent Zorbax SB-C18 (2.1 mm x 150 mm, 5 mm). The mobile phase was acetonitrile: 0.5% acetic acid (60: 40) and the flow rate was 0.3 mL x min(-1). The temperature of the column was 40.0 degrees C. In this condition, we established an analysis method to simultaneously determine the four components. A sensitive and specific liquid chromatography-mass spectrometric (LC-MS) method was developed and validated for the determination of artemisin in derivatives in mice plasma. The method we established has a linear range of 5-3 000 μg x L(-1) with a good sensitivity and specificity for all of the four components. This method is simple, rapid, accurate and suitable for the determination of the content of the four compounds.
科研通智能强力驱动
Strongly Powered by AbleSci AI