Determination of Trace Amount of Phenol and Aniline by Fluorescence Spectrophotometry in Natural Water

A new sepectrofluorimetric determination of phenol and aniline was porposed.The effluence of acidity,temperature and the stable time of the solution were discussed,and at the same time,the interference of coexistence substance was also discussed.The results demonstrated that both of the phenol and aniline had the maximum fluorescence intensity in the pH=6.86 Na2HPO4-KH2PO4 buffer solution at 25 ℃.The optimum excitation and emission wavelength of phenol and aniline were λex/λem=270/298 nm and λex/λem=280/340 nm,respectively.Under optimum conditions,the fluorescence intensities were linear with concentration of phenol and aniline in a range of 0.5 to 6.0 μg·mL-1.The detection limit of the method for phenol and aniline were 2.0 ng/mL and 3.6 ng/mL,respectively.The recovery was 98.0%~101.6%.This method was successfully applied to the determination of phenol and aniline in natural water.