The quantitative analysis method of cellulose,hemicellulose and lignin in roughage of cereal straw was established.The results showed that filtration was more easy to be operated as the neutral detergent was substituted by the pH2 detergent.The determining accuracy of hemicellulose and cellulose were increased as hemicellulose was decreased in ADF by the detergent of 2mol/L hydrochloric acid substituted the acidic detergent.In comparison with Van Soest method,the method of this paper has some property of easy and fast and high accuracy of quantitative.