Chemical constituents of Artemisia annua L.

Objective To study chemical constituents from the the leaves of Artemisia annua.Methods Compounds were isolated and purified by column chromatography over Sephadax LH-20 and silica gel column.Their chemical structures were elucidated on the basis of physicochemical properties and spectral data.Results Thirteen compounds were isolated,purified,and identified as:artemisinin(1),arteannuin B(2),3α-hydroxy-1-deoxyartemisinin(3),artemisinic acid(4),artemetin(5),chrysosplenetin(6),quercetagetin-3,7,3′,4′-tetramethyl ether(7),chrysosplenol D(5,3′,4′-trihydroxy-3,6,7-trimethoxyflavone,8),salicylic acid(9),domesticoside(2-O-βD-glucopyranosyl-4-O-methyl phloracetophenone,10),scopolin(11),β-sitosterol(12),daucostrol(13).Conclusions Compounds 14 belong to biosynthetic related sesquiterpenoid,and compound 10 is isolated from the Compositae family for the first time.