[Transfection of Fas(CD95) gene into HL-60 cell and its role in rhG-CSF-induced apoptosis].

OBJECTIVE: To explore the role of Fas gene in rhG-CSF-induced apoptosis. METHODS: Mediated by lipofection (DOTAP), Fas cDNA was transferred into HL-60 cells. In situ hybridization, Western blotting and FCM analysis were used to demonstrate the successful transfection. The apoptosis percentages of HL-60 and Fas transfected HL-60 cells were compared after coculturing with rhG-CSF at the same final concentration. RESULTS: After cocultured with rhG-CSF (10 ng/ml) for 96 hours, the apoptosis percentage of Fas transferred HL-60 cells was significantly higher than that of HL-60 cells, and the Fas expressions could be up-regulated by rhG-CSF. CONCLUSIONS: Fas is an apoptosis-induction oncogene, and rhG-CSF induces apoptosis of HL-60 cells through Fas signalling pathway.