Determination of hesperidin,honokiol and magnolol in Huoxiang Zhengqi Oral liquid by HPLC

OBJECTIVE To establish a method for the determination of effective components in Huoxiang Zhengqi Oral liquid by HPLC.METHODS Hesperidin,Honokiol and Magnolol were isolated by gradual elution with methanol-water,The chromatographic conditions were:DiamonsilTM C18 column,detecting wavelength at 283 nm,fiowing rate being 1mL·min-1 and the column temperature being 25℃.RESULTS A good Linearity of puerarin,Hesperidin,Honokiol and Magnolol was in the range of 0.1311～2.622μg、0.1728～3.4568μg、0.1751～3.5020μg respectively.CONCLUSION The method is simple,feasible and reproducible and can be used for the quality control of Huoxiang Zhengqi Oral liquid.