土壤水分
新烟碱
环境化学
益达胺
土工试验
环境科学
化学
基质(化学分析)
杀虫剂
色谱法
土壤科学
生态学
生物
作者
Eiji Watanabe,Nobuyasu Seike,Yutaka Motoki,Keiya Inao,Takashi Otani
标识
DOI:10.1016/j.ecoenv.2016.06.023
摘要
This study evaluated the applicability of commercially available kit-based enzyme-linked immunosorbent assay (ELISA) to simple, quick, and quantitative detection for three water-extractable (phytoavailable) neonicotinoid insecticides: dinotefuran, clothianidin, and imidacloprid in soils. ELISA showed excellent analytical sensitivity for determination, but with cross-reaction to structurally related neonicotinoid analogues, which might produce false positives. To analyze insecticides in soil samples of diverse physicochemical properties, they were extracted with water. The aqueous soil extracts were assayed directly with ELISA. No matrix interference was observed without additional dilution with water. Recovery experiments for the insecticides from aqueous soil extracts spiked at 2–10 ng/mL showed good accuracy (72–126%) and precision (<16%). Kit-based ELISAs were used to estimate soil–water distribution coefficients (Kd). Values estimated using this method showed positive correlation between organic carbon contents in soil and those for evaluated insecticides. Results indicate that the evaluated kit-based ELISA has applicability for simple, quick, and reliable detection of phytoavailable insecticides in soils and for estimating Kd values in soil.
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