High performance liquid chromatography gradient elution determination of ferulic acid in Naotongluo oral liquid

Objective To establish a high performance liquid chromatography(HPLC) gradient elution method for determination of ferulic acid in naoluotong oral liquid.Methods The HPLC systerm consisted of lichrospher C18 column(4.6 mm×250 mm,5 μm),methanol-0.1%phosphoric acid solution(adjusted PH to 4.15±0.02 with triehylamine)was used as amobile phase with gradient elution,the detector wavelength was set at 316 nm,the flow rate was 1.0 ml/min and the column temperature was 25℃.Results The linear range of ferulic acid was 8.112～64.896 μg/ml,r=0.9999.The average recovery was 98.15%,RSD was 1.79%(n=9).Conclusion The method is simple,accurate and reproducible,which can be used for determination of ferulic acid in Naotongluo oral liquid.