Loop-mediated Isothermal Amplification for the Detection of CaMV35S Promoter in Genetically Modified Maize

The objective is to develop a loop-mediated isothermal amplification method for detection of CaMV-35S promoter in Genetically Modified maize.Loop-mediated isothermal amplification(LAMP),a novel nucleic acid amplification method,was developed for the rapid detection of Genetically Modified maize.Cauliflower mosaic virus 35S(CaMV35S) promoter gene was amplified by a set of four primers that recognize six distinct sequences of the target.The optimized conditions for LAMP were studied using different Mg2+,dNTPs,Betaine and primers concentrations.Furthermore,the sensitivity of LAMP was tested comparing with PCR.The LAMP method can detect CaMV35S promoter from genetically modified maize and their test results were consistent with the results of conventional PCR method.LAMP assay results were found to be more sensitive than the conventional PCR.The LAMP assay is an extremely rapid,highly sensitive,specific method,and will be an effective tool for rapid detection of Genetically Modified maize.