Influence of Temperature Shock on the Numerical Variation of Scallop Hemocytes and Granulocytes

In this study,scallop(Chlamys farreri)was shocked with different temperatures by transferring them from 17℃to 11,23and 28℃,respectively,or air exposing them to 5,11,17and 23℃,respectively.The hemocytes and granulocytes were counted with enzyme-linked immunoadsorbent assay(ELISA)by using monoclonal antibody against hemocytes or granulocytes as the primary antibody separately.The percentage of hemocytes and granulocytes was determined with flow cytometry.The results showed that total hemocyte count(THC)increased significantly at 11and 23℃,but declined apparently at 28℃;while granulocyte count(GC)was significantly higher at 11℃than at 23and 28℃ with the minimum detected at 28℃.The percentage of granulocytes(PG)varied in the same way as GC did.GC and PG were always lower than those of control group.THC,GC and PG descended significantly after air exposure at 5,11and 23℃.It was found that THC,GC and PG of scallop air exposed at 5℃ were the highest,which were recovered when scallop were transferred back into seawater.No scallop survived air exposure at 23℃for 6h.In conclusion,the number of hemocytes and granulocytes was sensitive to temperature shock,being lower at 28℃than at other temperatures;air exposure significantly influenced hemocyte and granulocyte count,and the survival rate at 23℃ was the lowest.