An experimental study on supernate of osteoblast in treatment for glucocorticoid-induced osteoporosis in rats

Objective:To study the effectiveness of supernate of osteoblast on treatment of glucocorticoid-induced osteoporosis. Method:Thirty-six male SD rats which was glucocorticoid-induced osteoporosis were randomly divided into 3 groups (12 rats for each group): group A,given supernate of osteoblast 0.5ml/kg twice a week through intramuscular injection; group B,given culture medium RPMI 1640 0.5ml/kg twice a week through intramuscular injection; group C,given 0.9% natrium chloride 0.5ml/kg twice a week through intramuscular injection. The experiment lasted for 8 weeks,then the third lumbar vertebral body of all the rats was managed to do the measurement of bone mineral density,and the bone histomorphometric study of left femur was analyzed. Result:The value of bone mineral density in group A was significantly higher than that of group B(P0.01) and group C(P0.01). No significant difference was observed in bone mineral density between group B and group C(P0.05).In the bone histomorphometrical study the average of bone volume,osteoid surface,osteoid thickness,and osteoblast surface of group A were significantly higher than that of group B(P0.01) and group C(P0.01), while no difference was observed in the bone absorption surface of three groups(P0.05). Conclusion:Supernate of osteoblast can cure the osteoporosis induced by glucocorticoid.