单宁酸
脚手架
细胞粘附
细胞生长
纳米技术
材料科学
细胞
粘附
组织工程
再生(生物学)
过程(计算)
化学
生物医学工程
细胞生物学
生物化学
复合材料
计算机科学
有机化学
操作系统
生物
医学
作者
JiUn Lee,Miji Yeo,Won Jin Kim,YoungWon Koo,GeunHyung Kim
标识
DOI:10.1016/j.ijbiomac.2017.10.105
摘要
Cell-printing is an emerging technique that enables to build a customized structure using biomaterials and living cells for various biomedical applications. In many biomaterials, alginate has been widely used for rapid gelation, low cost, and relatively high processability. However, biocompatibilities enhancing cell adhesion and proliferation were limited, so that, to overcome this problem, an outstanding alternative, collagen, has been extensively investigated. Many factors remain to be proven for cell-printing applications, such as printability, physical sustainability after printing, and applicability of in vitro cell culture. This study proposes a cell-laden collagen scaffold fabricated via cell-printing and tannic acid (TA) crosslinking process. The effects of the crosslinking agent (0-3wt% TA) in the cell-laden collagen scaffolds on physical properties and cellular activities using preosteoblasts (MC3T3-E1) were presented. Compared to the cell-laden collagen scaffold without TA crosslinking, the scaffold with TA crosslinking was significantly enhanced in mechanical properties, while reasonable cellular activities were observed. Concisely, this study introduces the possibility of a cell-printing process using collagen and TA crosslinking and in vitro cell culture for tissue regeneration.
科研通智能强力驱动
Strongly Powered by AbleSci AI